Fig. 8
From: Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus
Expression of diterpenoid phytoalexin genes in WT-Az and MIR7695-Ac plants during blast infection. Plant material was treated as in Fig. 7. a-b RT-qPCR analysis of expression of (a) Phytocassane (OsCPS2, OsCYP71Z7, OsCYP76M6) and (b) momilactone (OsCPS4, OsMAS1) biosynthesis genes in rice leaves infected with M. oryzae. Data are mean ± SE (n = 3; each sample consisted of a pool of 3 individual leaves). Mock-inoculated (control, grey) and M. oryzae-infected (red) plants. c Heatmap showing log2 FC for each transcript and each time point (infected vs control) as determined from RT-qPCR values (a-b). Upregulated (purple) and downregulated (green). Dots indicate significant differences (infected vs control) (Student t test, p < 0.05). d Accumulation of diterpenoid phytoalexins, phytocassane E (PE), B (PB) and C (PC) (upper panels) and momilactone A (MA) and B (MB) (lower panels), in leaves of mock- and M. oryzae-infected plants. Each dot represents a biological replicate. FW, fresh weight. e RT-qPCR of expression of upstream diterpenoid biosynthetic genes (OsCPS2 and OsCPS4) in mock- and M. oryzae-infected leaves of rice plants treated under three different Fe supply conditions (low, sufficient, high). Data are mean ± SE (n = 3), each sample consisting of a pool of 4 individual leaves)