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Fig. 1 | BMC Plant Biology

Fig. 1

From: Investigations into a putative role for the novel BRASSIKIN pseudokinases in compatible pollen-stigma interactions in Arabidopsis thaliana

Fig. 1

Tissue-specific expression patterns of BKN1 and BKN2 in A. thaliana. a. Gene structures for the tandemly linked BKN1 and BKN2 genes. The promoter regions used for BKNp:GUS constructs are shown by blue arrows. b. RT-PCR analysis of different tissues show BKN1 and BKN2 expression in the stigmas. The double PCR bands observed for BKN1 is the result of the third intron not being properly spliced in the top band (determined by sequencing). This is also seen in the carpel RNA-Seq mapping data (Fig. 5).c-e. GUS staining of different tissues from BKN1p:GUS transgenic plants. GUS activity was specifically detected in stigmas across developmental stages in the inflorescence (c,d), and not in other tissues, including seedlings (e). f-h. GUS staining of different tissues from BKN2p:GUS transgenic plants. GUS activity was detected primarily in the floral abscission zones and stems (f, g). GUS staining was also seen in the petioles and the tips of leaves for about half of the samples (h). Scale bars are 1 cm for inflorescence images (right), and 500 μm for stage 12 flowers (centre) and 100 μm for seedlings (left)

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