Fig. 2

Pollen hydration assays for A. thaliana Col-0 bkn1 and bkn2 CRISPR deletion mutants. a. Structure of the tandemly linked BKN1 and BKN2 genomic region depicting the locations of CRISPR deletions. The bkn1 and bkn2 deletion mutants were generated separately and the CRISPR-generated deletion were confirmed by sequencing. The bkn1–3 mutant was then transformed with a CRISPR construct to delete the BKN2 gene resulting in the bkn1 bkn2 double mutants. b. Pollen hydration assays at 10 min post-pollination. Wild-type Col-0 pollen was applied to Col-0 stigmas and the different bkn mutant stigmas and left for 10 min. Pollen hydration results in a change in pollen grain diameter which was measured at 10 min post-pollination. Stigmas carrying a mutation in the BKN2 gene showed reduced Col-0 pollen hydration compared to Col-0 stigmas. n = 30 pollen grains per line. Letters represent statistically significant groupings of p < 0.05 based on a one-way ANOVA with a Duncan post-hoc test