Table 6 Primer pairs for RT-qPCR and PCR of CpMS1
Gene | Primer F | Primer R | Tm (C) |
|---|---|---|---|
Actin 2 | TTTCCAAGGGTGAGTATGATGAG | ACACAGGACACAAAAGCCAACTA | 58 |
EIF 1 | AGGCAGGCAAGAGAAGAT | TTCATACCGAGTAGCGATTC | 58 |
TBP 1 | GGTAGTAGTAGTTAGGTATGTG | GGCAATCTGGTCTCACTT | 58 |
CpMS1 | ATGGTCAGCTAAGCGAGTTG | CGGGTTTAAGCTACGACGAA | 58 |
CpMS1–1 | TTGGAAATTAACAAAACGAGAAA | TGGAGAGATTTCTTCAAAAGTTG | 55 |
CpMS1–2 | GCAATGGCCTCTTTGTTGTT | TCCTGCCTTCCAAAAGATCA | 55 |
CpMS1–3 | TCAACTCGCTTAGCTGACCA | TGTGAAGGTGGGTGTGATGT | 55 |
CpMS1–4 | AACCCACTTCGACTCCATTG | TTTCTCCATTTGCAGTGTTTCTT | 55 |