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Fig. 5 | BMC Plant Biology

Fig. 5

From: The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preference

Fig. 5

Localization of MsLAC1 in the cell wall compartment after transient expression in Nicotiana benthamiana leaf. Transient expression of MsLAC1-mCherry and LT16b-GFP (plasma membrane marker fused with GFP) in tobacco epidermal leaf cells after co-infiltration with Agrobacteria harboring the respective constructs. Upper panel shows cell wall association of MsLAC1, whereas the Lti6-GFP-labelled plasma membrane retracts. Lower panel shows a cell co-expressing Lti6b-GFP and a cytosolic mCherry control retracting with the protoplast. All pictures were taken 2 h after onset of plasmolysis (i.e. incubation in 30% w/v sucrose solution). Scale bars: 16 μm

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