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Table 1 Relative H, G, and S monomer contents in lignin from different Arabidopsis lines as determined by thioacidolysis

From: The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preference

Arabidopsis lines

H%

G%

S%

S/G

lac4–2 lac17

2.34 ± 0.51

54.41 ± 1.39a

43.24 ± 1.89 a

0.80 ± 0.06 a

pAtLAC17::Dummy-1

2.15 ± 0.10

54.55 ± 1.21 a

43.30 ± 1.24 a

0.79 ± 0.04 a

pAtLAC17::Dummy-2

2.44 ± 0.30

54.95 ± 1.38 a

42.62 ± 1.46 a

0.78 ± 0.05 a

pAtLAC17::MsLAC1–1

1.21 ± 0.05

60.92 ± 2.04 c

37.87 ± 2.08 b

0.62 ± 0.06 b

pAtLAC17::MsLAC1–2

0.94 ± 0.38

63.39 ± 0.57 bc

35.67 ± 0.29 b

0.56 ± 0.01 b

Col-0

1.28 ± 0.18

64.26 ± 1.01 b

34.46 ± 1.16 b

0.54 ± 0.03 b

p35S::Dummy-1

1.45 ± 0.45

64.28 ± 0.68 bc

34.27 ± 1.13 b

0.53 ± 0.02 b

p35S::Dummy-2

1.81 ± 0.26

64.36 ± 0.22 bc

33.83 ± 0.47 b

0.53 ± 0.01 b

p35S::MsLAC1–1

1.19 ± 0.72

70.63 ± 0.84 d

28.18 ± 0.17 c

0.40 ± 0.01 c

p35S::MsLAC1–2

1.77 ± 0.29

68.52 ± 0.59 d

29.71 ± 0.32 c

0.43 ± 0.01 c

  1. Percentages for H, G and S monomers, and S/G ratios. All measurements were repeated three times. One-way ANOVA (followed by Tukey test) was used to determine significant differences for G%, S% and S/G ratios at P < 0.05. Samples with significant statistic difference are labelled with different letters