Fig. 4

DEGs regulated by MC during root development in cotton. a The total number of up- and down-regulated DEGs in root middle region and root tip at three time points. b Venn diagram displaying DEGs and common genes upon soaking-seed treatment at three time points in root middle region and root tip. c Genes differentially expressed at all three time points in root middle region and root tip. Cotton seeds of K638 were treated with deionised water (Control) or 400 mg/L MC soaking-seed for 12 h. The roots sampled at 12, 48, and 72 h after treatment were subjected to RNA-seq analyses. “R” indicates the whole root, “M” indicates the root middle region (4 to 20–40 mm from the root tip), and “T” indicates the root tip region (0 to 4 mm from the root tip). The DEGs were controlled by FDR < 5% and |log₂Fc| ≥ 1. Values in boxes are log₂Fc. 3 biological replicates per treatment, 30 roots per replicate