Fig. 1From: The plant defensin gene AtPDF2.1 mediates ammonium metabolism by regulating glutamine synthetase activity in Arabidopsis thalianaAnalysis of the relative expression level of AtPDF family genes in the roots of A. thaliana under different treatments. The seedlings were treated with 1/4 plant nutrient solution for 18 d, N-starved for 3 d, and then treated with 0.2 mM KNO3, 2.25 mM KNO3, 10 mM KNO3, or 1.125 mM NH4NO3 for 6 h before roots were harvested separately for RNA analysis. a AtPDF1s. b AtPDF2s. Actin2 was used as the internal control in the quantitative real-time PCR. Bars displaying the same letters are not significantly different at P < 0.05 according to the least significant difference testBack to article page