Fig. 5

Identification of pdf2.1 mutants. a Schematic representation of the allelic transfer DNA insertion lines pdf2.1–1 (SALK_110286) and pdf2.1–2 (SALK_206700C).The white blocks represent 5′-3′ UTR and the line connected the two black blocks represents intron. b Identification of homozygote mutants by PCR screening and sequencing. The first column is wild type, and the second and third columns are screened homozygotes. L and 2 L represent the left primer of two mutants, R and 2R represent the right primer of two mutants, and B represents the inserted sequence. c Quantitative real-time PCR failed to detect intact AtPDF2.1 mRNA in pdf2.1 mutant plants. Actin2 was used as an internal control