Fig. 6From: The plant defensin gene AtPDF2.1 mediates ammonium metabolism by regulating glutamine synthetase activity in Arabidopsis thalianaFunctional disruption of AtPDF2.1 increases ammonium accumulation in shoot. a, b Ammonium concentration in Col-0, pdf2.1–1, and pdf2.1–2 plants grown in 1/4 plant nutrient solution for 21 d in (a) shoot and (b) root. c Ammonium concentration ratio of root to shoot. d AMT2.1 gene expression in roots of Col-0, pdf2.1–1, and pdf2.1–2. e Total N content of Col-0, pdf2.1–1, and pdf2.1–2 plants grown in 1/4 Plant Nutrient Solution for 21 d. Total N amount was obtained from pooled samples of six plants from each replicate. f Nitrogen use efficiency (NUE), NUE = total dry weight/total N content. Actin2 was used as an internal control. Data represent means ± SEs (n = 4). Bars displaying the same letters are not significantly different at P < 0.05 according to the least significant difference testBack to article page