Fig. 5

Expression of candidate genes with modest root-preferential expression. The expression of 13 genes highly expressed in one or two type(s) of root was analyzed by qRT-PCR. The expression of the maize Actin1 gene was used as an internal control. 6 DAS-root was used as the calibrator for calculating the expression of GRMZM2G375159, whereas leaf was used as the reference sample for the other genes. Three biological replicates were used, each with four technical replicates. Error bars indicate standard deviations