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Fig. 1 | BMC Plant Biology

Fig. 1

From: N-3-oxo-octanoyl-homoserine lactone-mediated priming of resistance to Pseudomonas syringae requires the salicylic acid signaling pathway in Arabidopsis thaliana

Fig. 1

Enhanced resistance of 3OC8-HSL-treated Arabidopsis against Pseudomonas syringae pv. tomato 3000. a, Symptoms of PstDC3000 infection on wild-type Arabidopsis pretreated with different N-acyl-homoserine lactone (AHL) compounds. Detached leaves from 5-week-old soil-grown Arabidopsis were pretreated with different AHL compounds at a concentration of 10 μM for 2 days prior to spray-inoculation with PstDC3000 (OD600 = 0.1). The disease symptoms were recorded 3 days after inoculation. Abbreviations: CK, wild-type Arabidopsis Col-0 inoculated with MgCl2; P., wild-type Arabidopsis Col-0 inoculated with PstDC3000. The remaining abbreviations indicate the following pretreatments: ET, ethanol; C4, C4-HSL; C6, C6-HSL; 3OC6, 3OC6-HSL; 3OC8, 3OC8-HSL; C8, C8-HSL; C10, C10-HSL; 3OC12, 3OC12-HSL; 3OC14, 3OC14-HSL; C12, C12-HSL. b, Proliferation of PstDC3000 in the leaves of hydroponically grown Arabidopsis plants pretreated at the roots with different 10 μM AHL (C4-C8) compounds for 48 h and with subsequent foliar spray-inoculation of PstDC3000 (OD600 = 0.1). Colony-forming units (CFUs) were counted at 72 h post-inoculation (hpi). c, Proliferation of PstDC3000 in the leaves of hydroponically grown Arabidopsis plants pretreated at the roots with different 10 μM AHL (C10-C1) compounds for 48 h and with subsequent foliar spray-inoculation of PstDC3000 (OD600 = 0.1). d, Effect of 3OC8-HSL concentration on induction of resistance against PstDC3000 in Arabidopsis. The roots of hydroponically grown plants were pretreated with different concentrations of 3OC8-HSL for 48 h prior to foliar inoculation with PstDC3000 (OD600 = 0.1). CFUs were counted at 72 hpi. e, Inhibitory effect of 3OC8-HSL on PstDC3000 growth in Arabidopsis. The leaves of hydroponically grown plants were inoculated with PstDC3000 (OD600 = 0.1) after 48 h of pretreatment with 10 μM 3OC8-HSL at the roots. CFUs were counted at the indicated hpi. Data represent the average values of three independent biological replicates ± standard deviation (SD). * indicate statistically significant differences (P < 0.05, Student’s t test). ** indicate statistically significant differences (P < 0.01, Student’s t test)

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