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Fig. 5 | BMC Plant Biology

Fig. 5

From: Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Fig. 5

Fluorescence in situ hybridization (FISH) analysis of TR77. Oligo-primers pSc119.2 (green) and pTa535 (red) were used as probes for wheat chromosomes during the mitosis metaphase in the root tip cells. a line TR77. b common wheat 7182. cP. huashanica. Wheat 3D and 5D chromosomes and chromosomes from two groups underwent double translocations to yield the 3DS-NsL.NsS and 5DL-3DS.3DL chromosomes as pointed by arrows. d Comparison of special chromosomes in common wheat 7182 and TR77. e Diagrammatic sketch of the two translocations, 3DS-NsL.NsS and 5DL-3DS.3DL, in TR77 to show breakage sites in each chromosome as pointed by arrows and chromosome rearrangement. Chromosomes were stained with 4′,6-diamidino-2-phenylindole (blue)

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