Fig. 2

Semi-quantitative RT-PCR analysis of AsHSP26.8a expression profile in leaf (left) and root (right) tissues under heat (37 °C), salt (200 mM NaCl), Drought, ABA (50 μM) treatment. Leaf samples were collected at 0, 0.5, 2 and 4 h after stress treatment and root samples were collected at 0 and 4 h after stress treatment. Total RNA was isolated using Trizol and 2 μg total RNA was used for reverse transcription by using reverse transcriptase II (NEB, USA). Creeping bentgrass ubiquitin gene AsUBQ was used as the endogenous control. The gel images were cropped to only retain PCR products. All original, full-length gel images were included as additional files in the supplementary materials. The PCR process: 95 °C for 5 min, 30 cycles of 95 °C for 30 s, 65 °C for 30 s, 72 °C for 30 s, for AsHSP26.8a; 95 °C for 5 min, 25 cycles of 95 °C for 30 s, 60 °C for 30 s, 72 °C for 30 s, for AsUBQ.