Fig. 3

Generation and molecular analysis of the AsHSP26.8a transgenic (TG) Arabidopsis thaliana.a, Schematic diagram of the AsHSP26.8a chimeric gene expression construct, p35S-AsHSP26.8a/p35S-bar. The AsHSP26.8a gene and an herbicide resistance gene, bar, are both under the control of the CaMV 35S promoter. RB, right border; LB, left border. b, PCR analysis of the AsHSP26.8a and bar gene in wild type (WT) and TG plants to detect transgene insertion in the Arabidopsis thaliana genome and transcription. Actin gene AtActin1 was used as the endogenous control. The gel images were cropped to only retain PCR products. All original, full-length gel images were included as additional files in the supplementary materials