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Fig. 3 | BMC Plant Biology

Fig. 3

From: Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Fig. 3

Interaction between gma-miR398c and its targets verified in transiently transformed mesophyll protoplasts. a Interaction vector [HBT-sGFP(S65T)-NOS–Targets] or [HBT-sGFP(S65T)-NOS–rTargets] inserted into mesophyll protoplasts derived from WT, OE-vector and OE-miR398c transgenic Arabidopsis plants. Bar = 50 μm; (b) Fluorescence ratio of mesophyll protoplast cells. Values are average of three replicates ± SD. Asterisks indicate significant difference applying Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). c Details regarding the gma-miR398c cleavage site in the target genes (GmCSD1a, GmCSD1b, GmCSD2a/b/c and GmCCS)

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