From: Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering
Cas9 Nuclease | Origin | PAM | Notes | References | Application to plant genome editing |
---|---|---|---|---|---|
SpCas9 | S. pyogenes | NGG | + | ||
NmCas9 | N. meningitidis | NNNNGMTT | [50] | - | |
StCas9 | S. thermophilus | NNAGAAW | + | ||
SaCas9 | S. aureus | NNGRRT, NNNRRT | + | ||
CjCas9 | C.r jejuni | NNNNRYAC | [55] | - | |
FnCas9 | F. novicida | No | + | ||
SpCas9-VQR | S. pyogenes | NGA | Altered PAM | + | |
SpCas9-EQR | S. pyogenes | NGAG | Altered PAM | + | |
SpCas9-VRER | S. pyogenes | NGCG | Altered PAM | + | |
SpCas9-NG | S. pyogenes | NG | Altered PAM | + | |
SpCas9-HF1 | S. pyogenes | NGG | High fidelity | + | |
eSpCas9 | S. pyogenes | NGG | High fidelity | + | |
HypaCas9 | S. pyogenes | NGG | High fidelity | [73] | - |
evoCas9 | S. pyogenes | NGG | High fidelity | [74] | - |
Sniper-Cas9 | S. pyogenes | NGG | High fidelity | [75] | - |
xCas9 | S. pyogenes | NG, GAA, GAT | Altered PAM, high fidelity | + |