Fig. 2

ZNC promoted hydrogen peroxide accumulation. ZNC promoted hydrogen peroxide accumulation in N. benthamiana. Hydrogen peroxide (up) and superoxide accumulation (down) were measured in leaves treated with 150 ng/mL of ZNC at different (0, 1, 2, 4, 6) hours post treatment (hpt) (n = 5). Quantification of hydrogen peroxide and superoxide levels in N. benthamiana treated with 150 ng/mL of ZNC at 0, 1, 2, 4, and 6 hpt. Data are shown as the mean (n = 5) ± SD. CAT, SOD, and APX were detected using qRT-PCR at various time intervals. Data are shown as the mean (n = 5) ± SD.qRT-PCR analysis of RbohA and RbohB expression at various time intervals. Data are shown as the mean (n = 5) ± SD. * indicates significant differences determined using the Student’s t-test (p < 0.05), ** indicates extremely significant differences determined using the Student’s t-test (p < 0.01).