Fig. 7

ZNC induced the antiviral activity of plants by increasing RNA silencing via SA pathway. a Photograph of wide type N. tabacum cv Samsun NN (up panel) and NahG transgenic N. tabacum cv Samsun NN (down panel) infiltrated with Agrobacterium harbouring PBI121-GFP after water (left panel) or ZNC (right panel) treatment, under an ultraviolet lamp at 3 dpi. b The relative expression levels of GFP from wild-type plants and NahG transgenic plants were detected by qRT-PCR. Data are shown as mean (n = 3) ± SD. * indicates significant differences determined using the Student’s t-test (p < 0.05). c Effect of ZNC on PVX-GFP accumulation in wild-type N. tabacum cv Samsun NN (up panel) and NahG transgenic N. tabacum cv Samsun NN (down panel). The photographs were taken under an ultraviolet lamp at 3 dpi. The plants were treated with water (left panel) or ZNC (right panel). d The relative expression relative quantity of PVX from wild-type plants and NahG transgenic plants were detected by qRT-PCR. Data are shown as mean (n = 3) ± SD. * *indicates significant differences determined using the Student’s t-test (N < 0.01).