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Table 7 Primers used in the qRT-PCR analysis

From: Gene expression and genetic control to cold tolerance during maize seed germination

Gene

Identification

 

Sequence 5′ - > 3’

GenBank Acession Number

Ubiquitin

Reference gene

F

AAGGCCAAGATCCAGGACAA

GRMZM2G027378_T01

R

TTGCTTTCCAGCGAAGATGA

SAD

Putative stearoyl-ACP desaturase

F

GGA TTT CCT CCC TGA CCC A

KU949326

R

GTC CAT GCC CTC GTC CAA A

ZmMPK5

Mitogen Activated Protein Kinase

F

ACTGATGGACCGCAAACC

AB016802

R

GGGTGACG AGGAAGTTGG

SOD

Superoxide Dismutase/Antioxidant

F

TGGAGCACCAGAAGATGA

X17565

R

CTCGTGTCC ACCCTTTCC

APX

Ascorbate Peroxidase/Antioxidant

F

TGAGCGACCAGGACATTG

EU969033

R

GAGGGCTTTGTCA CTTGGT

  1. (F) sequence of the forward primer and (R) sequence of the reverse primer