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Fig. 3 | BMC Plant Biology

Fig. 3

From: A modular cloning toolkit for genome editing in plants

Fig. 3

Assembly of level 1 crRNA expression modules to be used with respective Cas12a (Cpf1) and Cms1 nucleases. During the first step (a) annealed complementary oligos encoding the guide are inserted into a level 0 acceptor carrying the crRNA backbone using BpiI. During the second step (b), crRNA is fused with the respective Pol III promoter using BsaI

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