Fig. 6From: MYB transcription factor PdMYB118 directly interacts with bHLH transcription factor PdTT8 to regulate wound-induced anthocyanin biosynthesis in poplarExpression levels of ABGs in transgenic protoplasts transiently expressing PtrJAZ1. Protoplasts isolated from the leaves of WT and transgenic plants overexpressing PdMYB118 were used for the analyses. a Expression level of PtrJAZ1. b Expression levels of the ABG genes PtrCHS1, PtrCHI1, PtrF3H, PtrF3’H, PtrF3’5’H, PtrDFR2 and PtrANS1 respectively encoding chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, flavanone 3′-hydroxylase, flavonoid 3′5’-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase in Populus. Gene expression level in the WT was set to 1. WT, wild type protoplasts transfected with pGreenII62-SK; M, transgenic protoplasts transfected with pGreenII62-SK; M + J, transgenic protoplasts transfected with pGreenII62-SK-PtrJAZ1. Values (n = 3) are mean ± SD and different letters on the histograms indicate that the values differ significantly (one-way ANOVA; P < 0.01)Back to article page