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Fig. 5 | BMC Plant Biology

Fig. 5

From: Arabidopsis Chloroplast protein for Growth and Fertility1 (CGF1) and CGF2 are essential for chloroplast development and female gametogenesis

Fig. 5

Reduced fertility of the cgf1;cgf2 double mutants is due to compromised female gametogenesis or embryogenesis. a-g A representative silique from wild type a, cgf1–1b, cgf1–2c, cgf2d, cgf1–1;cgf2/+ e, cfg1–1/+;cgf2f,or cgf1–2;cgf2g. Arrowheads point at unfertilized ovules; arrows point at aborted seeds. h Seed set. Results are means ± SD (n > 10). Different letters indicate significantly different groups (OneWay ANOVA, Tukey’s multiple comparisons test, P < 0.05). i-l CLSM of a mature ovule from wild type i, cgf1–1;cgf2/+ j, cfg1–1/+;cgf2k,or cgf1–2;cgf2l. cc, central cell; ec, egg cell; es, embryo sac; sc, synergid cell. Dotted lines in j-l illustrate defective embryo sacs. The arrow in l points at a single nucleus. Numbers at the bottom indicate displayed ovules/total ovules examined. m-n Differential interference contrast (DIC) imaging of developing embryos in wild type m or in cgf1–2;cgf2n. Developing embryos are highlighted with lilac. Defective embryogenesis in cgf1–2;cgf2 is 9.4 ± 5.6% (n > 15). Bars = 1 mm for a-g; 20 μm for i-n

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