Fig. 7
Evaluation for CTV resistance of null segregates, using ImmunoStrip® for citrus tristeza virus (CTV) complete kit (Agdia). a PCR fragment amplified from five CTV lines, which have already infected in mother tree of ‘Kiyomi’ by specific primers, reported by Roy et al. (2010). M: 200 bp ladder, 1: EF1-α (884 bp), 2: T36 (836 bp), 3: CPG (positive control: 672 bp), 4: B165 (510 bp), 5: T3 (409 bp), 6: VT (302 bp), 7: T30 (206 bp). b) Photograph of rough lemon trees grafted with BC2–8 (left) and BC2–13 (right) scions on the top, with side a grafting of the CTV infected-mother tree, ‘Kiyomi scion. c Immunological detection of CTV. The presence of CTV test lines indicates that CTV propagation has occurred in the shoot and the absence the test lines indicates that CTV propagation was suppressed. K: mother tree of ‘Kiyomi’ scion, T: Trifoliate orange scion (CTV resistant), H: ‘Hyuganastu’ scion (CTV susceptible), 1: BC2–4 scion (CTV susceptible), 2: BC2–5 scion (CTV resistant), 3: BC2–8 scion (CTV resistant), 3: BC2–12 scion (CTV resistant), 5: BC2–13 scion (CTV susceptible), 6: BC2–14 scion (CTV susceptible)