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Table 2 Experimental and predicted values for secoiridoid and xanthone glycoside yield (%) optimized with central composite design (CCD)

From: Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

Run order

Treatment

Secoiridoid Yield (%)

Xanthone Yield (%)

SA (mM)

(A)

CS (mg L− 1)

(B)

Amarogentin (I)

Swertiamarin (II)

Mangiferin (III)

Experimental

Predicted

Experimental

Predicted

Experimental

Predicted

RSM

ANN

RSM

ANN

RSM

ANN

1

3.000

4.000

0.170

0.173

0.170

1.020

1.064

1.020

2.610

2.640

2.610

2

15.000

4.000

0.247

0.249

0.250

2.860

2.905

2.860

2.910

2.951

2.910

3

3.000

20.000

0.225

0.230

0.225

2.235

2.241

2.235

3.550

3.533

3.550

4

15.000

20.000

0.350

0.354

0.350

4.450

4.457

4.450

4.110

4.104

4.110

5

0.514

12.000

0.172

0.167

0.171

1.320

1.294

1.320

3.080

3.075

3.080

6

17.485

12.000

0.312

0.308

0.311

4.190

4.163

4.193

3.720

3.699

3.720

7

9.000

0.686

0.210

0.208

0.210

1.695

1.641

1.695

2.550

2.504

2.550

8

9.000

23.313

0.330

0.323

0.330

3.570

3.570

3.572

3.930

3.950

3.932

9

9.000

12.000

0.430

0.428

0.427

4.860

4.870

4.830

4.254

4.288

4.253

10

9.000

12.000

0.435

0.428

0.427

4.830

4.870

4.830

4.237

4.288

4.253

11

9.000

12.000

0.425

0.428

0.427

4.875

4.870

4.867

4.320

4.288

4.283

12

9.000

12.000

0.430

0.428

0.427

4.987

4.870

4.970

4.357

4.288

4.329

13

9.000

12.000

0.420

0.428

0.427

4.800

4.870

4.867

4.275

4.288

4.253