Fig. 5

Phylogenetic relationship of tobacco, N. sylvestris and N. tomentosiformis AQPs. Phylogenetic trees for each AQP sub-family were generated using the neighbour-joining method from MUSCLE alignments of nucleotide coding sequences. Confidence levels (%) of branch points generated through bootstrapping analysis (n = 1000). N. sylvestris (N. syl) and N. tomentosiformis (N. tom) AQPs are colour coded in blue and orange, respectively. Green stars indicate a loss of a parental gene in tobacco post-hybridisation; Blue and Red stars indicate gene loss events in N. sylvestris and N. tomentosiformis, respectively. Purple and Yellow stars indicate pre-hybridisation gene gain events in N. sylvestris and N .tomentosiformis, respectively