Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Role of phasiRNAs from two distinct phasing frames of GhMYB2 loci in cis- gene regulation in the cotton genome

Fig. 3

Expression pattern of phasing RNA triggering miRNA, their targeting genes and genes involved in tasiRNA biogenesis. a, qRT-PCR analysis of precursor forms of miRNAs including GhmiR828, GhmiR858 and their targeting gene GhMYB2. GhmiR390 and its targeting gene GhphasiRNA1 was served as the positive controls for phasiRNA generation. GhMYB109 was miR828 targeting gene and used as negative control for phasiRNA deriving. b, qRT-PCR analysis of genes involved in tasiRNA biogenesis, including AGO1, SGS3, RDR6, DCL4. The genes of AGO4, AGO7, DCL1, DCL2 and DCL3 were used as internal controls for gene redundancy in early ovules from − 1, 0, 1 and 3 days post anthesis (DPA), anthers, hypocotyls and seeds. Three biological replicates were used for each tissue

Back to article page