Fig. 2

Amino acid sequence alignment of putative nucleolar Pumilio proteins, APUMs and ChPUMs. a Alignment of the amino acid sequences of APUM23 and ChPUM2. b Alignment of the amino acid sequences of APUM24 and ChPUM3. The Puf domains are indicated with black and gray lines above the amino acid sequences, and the five residues in the 2nd α-helix of each Puf domain that potentially interact with RNA bases are indicated with red boxes. The thin gray lines in the C-R5 Puf domain represent unfolded chains. Basic amino acids conserved in the N-terminal Puf domains of APUM24 family proteins are boxed in purple. Arrows under the purple box indicate the amino acids that are not conserved in ChPUM3. Conserved aromatic amino acids of APUM24 family proteins are boxed in green, and the basic amino acids in the C-terminal region are boxed in blue