Fig. 7
Transcription capacity of the EDS1 promoter to drive GUS expression in the presence of melatonin and/or MET1. a Expression difference of the five selected genes in ‘Merlot’ berries at 48 HAT from RNA-Seq and qRT-PCR analyses. MET1, VIT_212s0035g01770; MET1B, VIT_212s0035g01755; SadMET, VIT_214s0006g02170; CMT2_1; VIT_216s0039g02470; CMT2_2, VIT_216s0039g02460. b Prediction of methylated cytosine in the EDS1 promoter (http://www.urogene.org/cgi-bin/methprimer2). O/E values indicate the ratio between the actual value and expected value of the CpG locus. The 800-bp region with a high CpG level indicated by the black box was used as the promoter of EDS1 (Peds) to produce the construct of Peds-35S miniGUS. c Histochemical analysis of the transcriptional capacity of Peds to drive GUS expression in grape calluses agroinfiltrated with different vector constructs: B1, control calluses; B2 and B4, Peds-35S miniGUS; B3, Peds-35S miniGUS and 35S::MET1; B5, Peds-35S miniGUS and 35S::MET1 with melatonin treatment; B6, Peds-35S miniGUS with melatonin treatment. d Gus activities of grape calluses infiltrated by Agrobacterium containing the B1-B6 constructs. e DNA methylation level of the EPS1 promoter, including endogenous DNA and DNA provided by Peds- 35S miniGUS, in calluses infiltrated by Agrobacterium containing the B4-B6 constructs