Fig. 6

DAB staining of leaves of OsSHMT transgenic and wild type A. thaliana (a) and the H₂O₂ content in the leaves (b). OsSHMT transgenic Arabidopsis thaliana and its wild-type were exposed to 0 °C for 12, 24 and 36 h, and leaves were sampled and stained with DAB, after which the chlorophyll in the leaves was dissolved using ethanol, leaving dark brown precipitate on the leave. Arrowheads indicate reddish-brown spots. Bar = 1 mm. The H₂O₂ content in the leaves was determined using an H₂O₂ determination kit (Solarbio Life Sciences)