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Fig. 6 | BMC Plant Biology

Fig. 6

From: MYB44 competitively inhibits the formation of the MYB340-bHLH2-NAC56 complex to regulate anthocyanin biosynthesis in purple-fleshed sweet potato

Fig. 6

Interaction verification of IbMYB340 with IbbHLH2, IbNAC56a or IbNAC56b and coregulatory site evaluation of the IbANS promoter. a Verification of the interaction of IbMYB340 with IbbHLH2, IbNAC56aor IbNAC56b via Y2H assays. b-d Verification of the interaction between IbMYB340 and IbNAC56aor IbNAC56b via firefly luciferase complementation assays. ** indicates a significant difference at the P < 0.01 level. e Schematic of the IbANS promoter. The prediction of the cis-acting elements in the 1228-bp promoter region of IbANS was performed using the PlantCare database [32]. f Validation of the activation effect by cotransformation of IbMYB340, IbbHLH2, and IbNAC56 on the IbANS promoter using a dual-luciferase assay in tobacco leaves. The ratio of Luc to Ren indicates that TFs activate the promoter activity of IbANS. g Y1H assays for IbMYB340, IbNAC56a, and IbNAC56b for the IbANS promoter. The prey vectors pGADT7-IbMYB340/IbNAC56a/IbNAC56b were transformed into Y1H Gold cells harboring pAbAi-IbANS1/2/3 and then tested on SD/−Ura/−Leu/AbA400 plates

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