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Fig. 7 | BMC Plant Biology

Fig. 7

From: MYB44 competitively inhibits the formation of the MYB340-bHLH2-NAC56 complex to regulate anthocyanin biosynthesis in purple-fleshed sweet potato

Fig. 7

Functional analysis of the IbMYB340 and IbMYB44 genes using transient expression assays in tobacco leaves and interaction verification of IbMYB44 with IbMYB340, IbNAC56a or IbNAC56b in vivo. a The phenotypes of tobacco leaves on the 7th day after infiltration; the letters ‘a’ ~ ‘h’ represent the ratio of Agrobacteria harboring the IbMYB340 and IbMYB44 constructs that were coinfiltrated into young tobacco leaves. Scale bar = 1 cm. b Transcriptional effects of coinfiltration of IbMYB340 and IbMYB44 at different ratios on IbANS promoter activity. c Anthocyanin contents in transformed leaves of tobacco. FW, fresh weight. d-e The color parameter L* and a*/b* ratio values were used to indicate phenotypic changes. The values are presented as the means ± SDs (n = 3), and the uppercase and lowercase letters represent significant differences in ratios at P < 0.01 or P < 0.05, respectively. f Verification of the interaction between IbMYB44 and IbMYB340, IbNAC56a,or IbNAC56b via Y2H assays. g Verification of the interaction between IbMYB340 and IbMYB44 via firefly luciferase complementation assays. h-i Verification of the interaction between IbMYB44 and IbNAC56a or IbNAC56b via firefly luciferase complementation assays

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