- Research article
- Open Access
Interactive effects of elevated CO2 concentration and combined heat and drought stress on tomato photosynthesis
BMC Plant Biology volume 20, Article number: 260 (2020)
Extreme weather events are predicted to increase, such as combined heat and drought. The CO2 concentration ([CO2]) is predicted to approximately double by 2100. We aim to explore how tomato physiology, especially photosynthesis, is affected by combined heat and drought under elevated [CO2] (e [CO2]).
Two genotypes, ‘OuBei’ (‘OB’, Solanum lycopersicum) and ‘LA2093’ (S. pimpinellifolium) were grown at a [CO2] (atmospheric [CO2], 400 ppm) and e [CO2] (800 ppm), respectively. The 27-days-old seedlings were treated at 1) a [CO2], 2) a [CO2] + combined stress, 3) e [CO2] and 4) e [CO2] + combined stress, followed by recovery. The PN (net photosynthetic rate) increased at e [CO2] as compared with a [CO2] and combined stress inhibited the PN. Combined stress decreased the Fv/Fm (maximum quantum efficiency of photosystem II) of ‘OB’ at e [CO2] and that of ‘LA2093’ in regardless of [CO2]. Genotypic difference was observed in the e [CO2] effect on the gas exchange, carbohydrate accumulation, pigment content and dry matter accumulation.
Short-term combined stress caused reversible damage on tomato while the e [CO2] alleviated the damage on photosynthesis. However, the e [CO2] cannot be always assumed have positive effects on plant growth during stress due to increased water consumption. This study provided insights into the physiological effects of e [CO2] on tomato growth under combined stress and contributed to tomato breeding and management under climate change.
The atmospheric CO2 concentration (a [CO2]) is predicted to be up to 443–541 ppm in 2050 and to be the double of the current concentration by 2100 . The a [CO2] has already rapidly peaked 414.8 ppm within the average of May, 2019 (https://scripps.ucsd.edu/programs/keelingcurve/2019/06/04/carbon-dioxide-levels-hit-record-peak-in-may/). The effects of elevated [CO2] (e [CO2]) on plant were extensive from different aspects. For instance, it is known that e [CO2] can increase intercellular [CO2] (Ci) and net photosynthetic rate (PN) and decrease the stomatal conductance (gs), contributing to the increase of water use efficiency in plants [2, 3]. The e [CO2] induced stomatal closure or decreased stomatal density with the generation of reactive oxygen species, ABA receptors and ABA itself as prerequisite in plants  and it generally improves salicylic acid biosynthesis but represses jasmonic acid pathway in tomato . Moreover, the e [CO2] upregulated 22 genes but downregulated 14 genes mainly playing roles in photosynthesis and development on leaves of sugarcane . The e [CO2] can reduce the transcriptional alteration and delay leaf senescence in birch .
Co-occurrence of different abiotic stresses lead to complex responses of plants that can not be deduced from single stresses . Among the abiotic stresses, increased temperature and water deficit are the two main constraints to agricultural production that often co-occur in the field [9, 10]. The e [CO2] in atmosphere contributes to global warming and induces changes in precipitation patterns, which leads to water scarcity in many areas . Along with the e [CO2], global change predicts more frequent abiotic stresses such as heat and drought in future climate, which will complicate the effect of e [CO2] on plants [12, 13]. To date, most researches have focused on the response of plants to e [CO2] at single abiotic stresses. For instance, Jiang et al. (2016) showed that the e [CO2] mitigated the damage of moderate and severe drought stress . The e [CO2] could enhance water-use efficientcy and improve plant water relation through decreasing leaf gs at drought stress [14, 15]. The thresholds for fraction of transpirable soil water when PN and gs began to drop were lower in plants at e [CO2] than a [CO2] along with progressive soil drying, indicating the [CO2] hindered gas exchange response to drought . On the other hand, the e [CO2] generally alleviated the heat stress damage in terms of plant physiology, such as photosynthesis, hydrogen peroxide (H2O2) generation and stomatal movement characteristics [13, 17].
However, it is important and meaningful to consider the co-occurrence of heat and drought for future crop breeding and management . The crosstalk between e [CO2] and combined drought and heat has been investigated in Arabidopsis thaliana [19, 20], Triticum aestivum [21, 22], Brassica napus  and C3 grassland . The stress-mitigating e [CO2] effect operates in Arabidopsis thaliana by up-regulating the antioxidant defense system and decreasing photorespiration  and reducing the negative influence on sugar and amino acid metabolism with less extent of metabolic alteration . Fitzgerald et al. (2016) showed that the e [CO2] enhanced wheat yield in semi-arid environments with heat waves , while de Oliveira et al. (2013) indicated that the effect on wheat biomass and yield was dependent on temperature . The e [CO2] improved plant water relations, contributing to alleviate the adverse effect of combined stress on photosynthetic rate at saturating light . The e [CO2] alleviated the negative effects of droughts and heat waves on ecosystem net carbon uptake of C3 grassland .
Tomato is a globally important crop and a model crop for plant research. The e [CO2] broadly affects the physiology, metabolism, gene expression and yield of various plants including tomato [3, 25, 26]. These effects induced by the e [CO2] have been proven to increase the tolerance of tomatoes to individual heat and drought [13, 27]. However, the effects of e [CO2] is not yet fully understood in tomato when being interacted with combined heat and drought. This study aims to is to explore the physiological responses of tomato plants at combined heat and drought to e [CO2]. Two genotypes (S. lycopersicum and S. pimpinellifolium) with different heat susceptibilities were applied to a [CO2] + combined stress, e [CO2] and e [CO2] + combined stress followed by a recovery stage (Fig. 1). Our hypothesis was that (1) the e [CO2] alleviate the damage of combined heat and drought stress on tomato; (2) the tomato grown at the e [CO2] recover faster than control condition; (3) the effect of CO2 concentration differ between genotypes with different heat sensitivities. Our results will provide knowledge on the response of cultivated and wild tomatoes to combined stress in future CO2-enriched environmental conditions. This is important to efficiently predict the complex effects of climate changes on crops. We will also help to manage the challenges of future food security under climate changes accompanied by increased population through tomato breeding using tolerant wild species.
The PN and gs of ‘OB’ and ‘LA2093’ at a [CO2] was significantly lower than that at e [CO2] at control condition (Figs. 2A, B and 3A, B). Combined heat and drought stress significantly decreased the PN of ‘OB’ and ‘LA2093’ plants at both a [CO2] and e [CO2] (Figs. 2A and 3A). The PN of ‘OB’ and ‘LA2093’ plants at a [CO2] during the recovery stage showed no significant difference from the controls (Figs. 2A and 3A). The PN of ‘OB’ plants at e [CO2] during recovery were significantly higher than that during combined stress but lower than control, while that of ‘LA2093’ increased to higher level during recovery from HS (heat stress) and showed no significant difference with control (Figs. 2A and 3A). The gs of ‘OB’ and ‘LA2093’ plants at e [CO2] during combined stress, and recovery from DS (drought stress) and HS was significantly lower that under control, while that of the plants at a [CO2] showed no significant difference (Figs. 2B and 3B). The e [CO2] increased the gs of the plants from both genotypes as compared with a [CO2] (Figs. 2B and 3B). The E (transpiration rate) of ‘OB’ and ‘LA2093’ at a [CO2] during combined stress and recovery from DS were significantly higher than control (Figs. 2C and 3C). Nevertheless, the E and Ci of ‘OB’ plants at e [CO2] during combined stress and recovery from HS and ‘LA2093’ plants at e [CO2] during combined stress and recovery from DS were significantly lower than control (Figs. 2C, D and 3C, D). Moreover, leaf temperature of ‘OB’ and ‘LA2093’ at both a [CO2] and e [CO2] during combined stress and recovery from DS were significantly higher than the respective controls (Figs. 2E and 3E).
The combined stress significantly decreased the Fv/Fm (maximum quantum efficiency of photosystem II) of ‘OB’ at e [CO2] and ‘LA2093’ at both a [CO2] and e [CO2] (Fig. 4). The Fv/Fm of both genotypes during the recovery from DS was significantly lower than the respective controls without stress regardless of CO2 concentration (Fig. 4). By comparison, there was no significant difference in Fv/Fm between all the treatments during the recovery from HS (Fig. 4).
The stomata and pore length and stomata area of ‘OB’ at e [CO2] was higher, while those at e [CO2] + combined stress was lower as compared with a [CO2] and a [CO2] + combined stress (Fig. 5A, C, E). The length and width of stomata and pore as well as stomata area of ‘LA2093’ at a [CO2] + combined stress was smallest among the four treatments (Fig. 5A, B, C, D, E). The stomatal density of ‘OB’ at e [CO2] + combined stress was highest than the other three treatments (Fig. 5F).
Combined stress with e [CO2] showed distinct effects on the chlorophyll and carotenoid content of the genotypes. The chlorophyll a and b content in the leaves of ‘OB’ at e [CO2] with or without combined stress significantly increased than the control at a [CO2] (Fig. 6A, B). However, the chlorophyll a and carotenoid content in the leaves of ‘LA2093’ at a [CO2] + combined stress, e [CO2] and e [CO2] + combined stress were significantly lower than control at a [CO2] (Fig. 6A, C). The combined stress significantly decreased the chlorophyll a/b in the leaves of both genotypes regardless of CO2 concentration (Fig. 6D).
Similar to the changes in chlorophylls and carotenoid content, the effects of combined stress and e [CO2] on carbohydrates content are genotype dependent. The glucose content in the leaves of ‘LA2093’ at a [CO2] + combined stress was significantly lower than the other treatments (Fig. 7A). The fructose content in the leaves of ‘LA2093’ at a [CO2] + combined stress significantly decreased as compared with e [CO2] and e [CO2] + combined stress (Fig. 7B). These suggested that the e [CO2] had a stress-mitigating effect on the monosaccharide accumulation of ‘LA2093’. The e [CO2] + combined stress significantly increased the sucrose content in the leaves of ‘OB’ in comparison with that at a [CO2] (Fig. 7C). The starch content in the leaves of ‘OB’ and ‘LA2093’ at e [CO2] was significantly higher than that at a [CO2] and a [CO2] + combined stress (Fig. 7D).
The leaf FW (fresh weight) and DW (dry weight) of ‘OB’ at e [CO2] were significantly higher than a [CO2] regardless of stress after both stress and recovery stage (Fig. 8). Both genotypes grown at e [CO2] + combined stress showed wilted leaves and weak growth during the stress; however, the symptoms disappeared after the recovery (Fig. 9). The root did not show visible difference between the four treatments during stress and recovery (Fig. 9).
In recent years, the interaction between e [CO2] and abiotic stress conditions has drawn more attention . The predicted increase in [CO2] is expected to lead to more frequent extreme high temperature and/or drought episodes . Reduced irrigation together with e [CO2] could improve tomato fruit quality at high N supply . The e [CO2] enhanced water use efficiency and reduced the negative influence of heat stress on tomato partially due to e [CO2]-induced respiratory burst oxidase 1/RBOH1-dependent H2O2 accumulation and stomatal closure . Our previous results showed that combined heat and drought lead to unique physiological responses of tomatoes as compared with single stress . Thereby, the interaction between the e [CO2] and combined stress on tomato physiology is complex, which cannot be determined by the effect of e [CO2] and one stress only. However, little is known about whether the e [CO2] can change the response of tomatoes at combined stress such as heat and drought.
The physiological and transcriptional response in Arabidopsis thaliana showed that photosynthesis, as a fundamental biological process, was a major target of climate extreme like combined heat waves and drought . The e [CO2] increased the PN of both genotypes in comparison with a [CO2] without combined stress, in agreement with the previous studies showing that the plants grown under e [CO2] have higher photosynthetic capacity [17, 25]. Zinta et al. (2014) showed that the combined stress inhibited the PN of Arabidopsis thaliana regardless of [CO2] . The lower PN of tomato at a [CO2] is due to non-stomatal factor since the gs and Ci were unaffected, while the PN of tomato at e [CO2] accompanied by drops in both gs and Ci was attributed to stomatal factor during combined stress (Fig. 10), according to the description of Von Caemmerer and Farquhar (1981) . Surprisingly, the e [CO2] increased the gs of both tomato genotypes as compared with a [CO2] as the gs has been commonly reported to decrease when plants were grown at e [CO2] [13, 32]. The increased stomatal area due to the elongation of stomatal as indicated by longer length and unchanged width of stomatal and pore in plants at e [CO2] corresponded to the result of gs (Fig. 10). By comparison, Chavan et al. (2019) found no difference in the gs of wheat plants grown and measured at a [CO2] and e [CO2, 17], while Zinta et al. (2014) reported the gs of Arabidopsis thaliana plants grown at e [CO2] increased at unstressed condition . However, the reason behind for the inconsistent change of gs caused by e [CO2] need to be further clarified, which could depend on plant species or developmental stage and be linked to limitation in water or nutrients. Moreover, the e [CO2] alleviated the reduction in PN in heat-tolerant tomato under combined stress since the PN at e [CO2] + combined stress was higher than a [CO2] + combined stress for ‘LA2093’. This e [CO2]-alleviating effect on plant grown at combined heat and drought has been reported in Arabidopsis thaliana [19, 20], Triticum aestivum  and C3 grassland . We suggest that one of the key factors that influenced the alleviation of e [CO2] is the sensitivity of the genotype to stress conditions (Fig. 10). More importantly, the PN of the heat-tolerant tomato plant at e [CO2] fully recovered from the combined stress, while that of ‘OB’ did not, although the PN of both genotypes at a [CO2] fully recovered. Thereby, the differential genotypic response to the e [CO2] effect on the PN of plants happened both during stress and recovery (Fig. 10). The underlying genotypic difference need to verify in further studies, particularly for crop improvement to adapt to future e [CO2] atmosphere.
The Fv/Fm has been widely and successfully applied to identify stress damage on plants’ photosynthetic apparatus . The e [CO2] did not alleviate the negative effects caused by short-term combined stress (12 h) as indicated by the decreased Fv/Fm in both genotypes at e [CO2] + combined stress. Accordingly, the alleviating effect of e [CO2] on Fv/Fm was observed in plants exposed to a longer duration (7 days) to combined stress compared with 2 day and 4 days of exposure as shown by Zinta et al. (2014) . The tomato plants did not fully recover and continued to deteriorate after watering, as indicated by the consistent low Fv/Fm. This result could be explained by the longer duration of heat stress, as the high temperature persisted even though the plants were irrigated. However, the plants fully recovered in terms of Fv/Fm after being exposed to control temperature.
Zinta et al. (2014) showed there were no difference in chlorophylls content of Arabidopsis thaliana induced by e [CO2] or combined stress for 4 days, but combined stress for 8 days decreased the chlorophylls content regardless of [CO2] . The chlorophyll a and b content of ‘OB’ at e [CO2] regardless of combined stress was higher than a [CO2] and combined stress decreased the content of chlorophyll a of ‘LA2093’ indicating that content depended on genotypes. The chlorophyll a/b of both genotypes at combined stress decreased in regardless of [CO2], indicating an oxidative stress damage .
In accordance with Li et al. (2013)  and Zinta et al. (2018) , the e [CO2] enhanced the starch accumulation of plants without stress conditions. The glucose, fructose and starch of ‘LA2093’ at e [CO2] + combined stress was higher than that at a [CO2] + combined stress. Together with the higher sucrose content at e [CO2] + combined stress than a [CO2], it could indicate that e [CO2] could alter the carbohydrate metabolism and induce carbohydrates accumulation by enhancing photosynthesis without/with combined stress [19, 35].
The leaf FW and DW of ‘OB’ treated at e [CO2] and e [CO2] + combined stress was higher than that at a [CO2] and a [CO2] + combined stress during both stress and recovery (Fig. 8), but there were no difference in stem FW and DW (Data not shown). Our finding corresponds to the results by Pazzagli et al. (2016) , Chavan et al. (2019) , Fitzgerald et al. (2016)  and Ainsworth and Long (2005) , showing that the e [CO2] enhance the biomass production of plants. This could be partially explained by increased photo-assimilates in source leaves of tomato grown at e [CO2] as a consequence of increased PN. In addition, the response of biomass accumulation to e [CO2] differed between the tomato genotypes. The ‘LA2093’ showed no significant difference in biomass at e [CO2], which could be due to increased leaf dark respiratory rate accompanied by high carbohydrates availability to increase glycolysis and tricarboxylic acid (TCA) cycle flux .
Both genotypes grown at e [CO2] were more sensitive to combined stress than those grown at a [CO2] (Fig. 9). This correspond to the fact that combined stress lead to a higher drop in PN of the plants grown at e [CO2] than those grown at a [CO2]. The explanations could be that the plants at e [CO2] generally had more biomass accumulation and higher gs as compared with a [CO2], leading to higher transpiration and more irrigation requirement. This created a situation that the water depletion of tomatoes at e [CO2] happened faster and the growing media become drier  and thereby the severity of water deficit at combined stress was higher. The gs at drought of Fagus sylvatica L. grown from seed for two seasons at e [CO2] was higher instead of lower than a [CO2], causing faster soil drying . Moreover, the gs was less sensitive to induce ABA accumulation in tomato at e [CO2] and thereby ABA signialing could not play role in stomatal closure at water deficit condition . There may be harmful influences of the e [CO2] for some species on plant-water relations in some cases . Hence, the e [CO2] cannot be always assumed have positive effects on plant growth due to other limiting factors.
The climate changes including e [CO2], heat and drought interact with each other under field conditions, which can lead to effects that are remarkably different from single stress exposures. As compared with a [CO2], the e [CO2] increased the PN of both tomato genotypes. However, the combined stress inhibited the PN regardless of [CO2] and genotype. The Fv/Fm remained lower during recovery from drought, but fully recovered after being exposed to control temperature. The e [CO2] environment could enhance the biomass production of plant, but the magnitude of the effects depended on plant genotypes. Genotypic difference was observed in e [CO2] effect on the PN, carbohydrate accumulation, pigment content and dry matter accumulation. The e [CO2] cannot be always assumed have positive effects on plant growth during stress conditions due to its deleterious effects on plant-water relations. This study offers insights into the understanding of physiological responses of tomatoes to the interactive effects of future climate factors, which is vital to identify resilient crops for future climate regimes.
The seeds from tomato ‘OuBei’ (‘OB’) (Solanum lycopersicum) were acquired from Northern Nuohua co. LTD, China. The seeds of wild heat-tolerant tomato ‘LA2093’ (S. pimpinellifolium)  were provided by Nanjing Agricultural University. The seeds were sown in plastic pots (9 cm height, 11 cm-diameter) in two separate compartments (compartment 1: 400 ppm [CO2]; compartment 2: 800 ppm [CO2]) of a greenhouse at the Department of Food Science, Aarhus University, Aarslev, DK (55.30 N, 10.44E). There were 36 plants per genotype per compartment. The commercial substrate used was Pindstrup 2 (Pindstrup Mosebrug A/S, Ryomgaard, Denmark). The seedlings were grown at approximately 23/16 °C (day/night), relative humidity of 40–60% and 150–300 μmol m− 2 s− 1 photosynthetic photon flux density (PPFD). The nutrition solution (pH = 6, EC = 2.18, K = 275 ppm, N = 191 ppm and P = 35 ppm) was applied to 18-day-old seedlings every day. Six days later, the 24-day-old seedlings in compartment 1 were moved to climate chamber 1 with the level of [CO2] (400 ppm), while those in compartment 2 were moved to climate chamber 2 with the level of [CO2] (800 ppm). The temperature was set at 25 °C for 15 h during daytime (05:00–20:00) and at 20 °C for 9 h during nighttime (20:00–05:00) in both chambers. The setting of relative humidity and PPFD in both chambers was 60% and 300 μmol m− 2 s− 1 PPFD provided by LED (FL300 sunlight, Fionia Lighting, Søndersø Denmark), respectively. The nutrition solution was applied every day by flooding the bench for about 10 min.
The plants were acclimated in the chambers for 3 days and then the 27-day-old plants in chamber 1 and 2 were equally distributed to chamber 3 and chamber 4, respectively. The four treatments were imposed: (1) a [CO2] in chamber 1, 400 ppm [CO2] + 25/20 °C + irrigation; (2) e [CO2] in chamber 2, 800 ppm [CO2] + 25/20 °C + irrigation; (3) a [CO2] + combined stress in chamber 3, 400 ppm [CO2] + 35/30 °C + no irrigation and (4) e [CO2] + combined stress in chamber 4, 800 ppm [CO2] + 35/30 °C + no irrigation. There were 18 plants per treatment per genotype. Based on our preliminary test, the irrigation was stopped 4 hours before the high temperature since it took time to make drought stress happen after withdrawing the irrigation, while heat stress happen quickly (10–20 min) after increasing the temperature. Thereby, the irrigation was withdrawn at 16:00 and the temperature was increased to 30 °C at 20:00 for the plants at combined stressed. The combined stress was regarded to begin at 20:00 on day 0. After 20 h of the treatments when the plants at combined stress has shown macroscopic stress phenotype, the irrigation was restarted at 16:00 on day 1 in the same way as the control. This stage from 16:00 on day 1 until 8:00 on day 2 (16 h) was defined as the recovery from DS. The temperature was changed to 25/20 °C from 8:00 until 14:00 on day 2 (6 h) for the plants at combined stress. This stage was defined as the recovery from HS. The experimental time line of treatments was shown in Fig. 1.
The PN, gs, E, Ci and leaf temperature were measured in the first fully expanded leaf from the top of the plants with four replications. The measurements were taken at 8:00 on day 1 after 12 h of the stress treatments, at 8:00 on day 2 after 16 h of recovery from DS and 12:00 on day 2 after 4 h of recovery from HS using portable photosynthesis system (CIRAS-2, PP Systems, Amesbury, USA). The light level, temperature and CO2 concentration settings of the cuvette during the measurements corresponded to the respective growing conditions. The records were taken when the parameters were considered stable. The average of the six records within the last 1 min were regarded as the results.
Maximum quantum efficiency of photosystem II (Fv/Fm)
The Fv/Fm in the first fully expanded leaf from the top of the plants was measured using Handy PEA (Hansatech Instrument, King’s Lynn, England). The leaf was dark adapted for 20 min with a leaf clip. The measurements with four replications were taken at 10:00 on day 1 after 14 h of the stress treatments, at 10:00 on day 2 after 18 h of recovery from DS and 14:00 on day 2 after 6 h of recovery from HS.
Original imprints were obtained by evenly painting the impression material (elite HD+, Zhermack, Badia Polesine, Italy) to the leaf surface. The imprints were transferred by applying nail varnish to the original imprints. Image data was acquired using a Nikon AZ100 microscope (Nikon Corp., Tokyo, Japan) equipped with a Nikon DS-Fi1 camera. There were four imprints from the abaxial leaf surface of the four plants per treatment. For each imprint, four photos were taken at different locations in the slide. Stomata number was counted for each picture excluding those cut by the picture border and stomatal density was calculated per area. For all stomata, stomatal length, stomatal width, pore length, pore width and stomata area (the area of the rectangle encasing the stomata) were investigated.
Chlorophyll and carbohydrate content
The first fully expanded leaf from the top were harvested during the stress treatments for 20 h. One leaf disk (2.96 cm2) per plant were punched using a cork-borer for chlorophyll content measurements with three replications per treatment. The leaf samples were freeze-dried and grounded before carbohydrate content measurements. Chlorophyll and carbohydrate content were determined according to Zhou et al. (2015) .
Fresh and dry weight (FW and DW) of leaf
The leaf FW and DW of the plants with three replications during the stress and recovery was determined using an analytical balance. The leaf DW were measured after putting the fresh samples at 80 °C in a constant flux oven for 48 h.
The photosynthesis and Fv/Fm data of each cultivar at a [CO2] and e [CO2] during the control condition, combined stress, recovery from DS and recovery from HS were analyzed by one-way Analysis of variance (ANOVA) (Duncan’s post hoc test) using SPSS 16.0 (SPSS Inc. Chicago, IL). The stomatal, pigment and carbohydrate content as well as biomass data of each cultivars at a [CO2] and a [CO2] + combined stress, e [CO2] and e [CO2] + combined stress were analyzed by one-way ANOVA (Duncan’s post hoc test). To perform the one-way ANOVA in SPSS, choose ‘Analyze’ → ‘General Linear Model’ → ‘Univariate’ after inputting data based on the methods from DeCoster and Claypool (2004) . The significant levels were at 0.05.
Availability of data and materials
The datasets during or analyzed during the current study available from the corresponding authors on reasonable request.
- a [CO2]:
atmospheric CO2 concentration
Analysis of variance
- C i :
intercellular CO2 concentration
- E :
- e [CO2]:
elevated CO2 concentration
- Fv/Fm :
Maximum quantum efficiency of photosystem II
- g s :
- H2O2 :
- P N :
Net photosynthetic rate
Photosynthetic photon flux density
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We appreciated the help from Eje Mattis Röndahl as well as Kaj Ole Dideriksen for the management of plant materials and climate chambers.
The work got financial support from the funding of Aarhus University Research Foundation (AUFF grant, 30379) and the funders acted no role in performing experiment and manuscript preparation.
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Zhou, R., Yu, X., Wen, J. et al. Interactive effects of elevated CO2 concentration and combined heat and drought stress on tomato photosynthesis. BMC Plant Biol 20, 260 (2020). https://0-doi-org.brum.beds.ac.uk/10.1186/s12870-020-02457-6
- Elevated CO2 concentration
- Combined heat and drought
- Plant physiology