Fig. 8

Expression profiles of CsHsf genes under heat stress conditions (38 °C for 4 h) in tea plants (C. sinensis cv. ‘Longjing-changyecha’) leaves pre-treated with exogenous calcium (20 mM). a Fragments per kilobase of transcript per million fragments mapped (FPKM) values [37] and HemI [56] were used to create the heat map. The color bar represents the normalized FPKM values (log2-transformed), and the black represents no expression detectable. Detailed FPKM values are listed in Additional file 7: Table S6. b Verification of the RNA-seq results of eight CsHsf genes by qRT-PCR analysis. Three biological replicates and three technical replicates were performed for the experiment. The qRT-PCR data was analyzed by ANOVA followed by Fisher’s LSD multiple comparison tests and ** represents significant differences at P < 0.01