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Fig. 5 | BMC Plant Biology

Fig. 5

From: Transcription factor TabHLH49 positively regulates dehydrin WZY2 gene expression and enhances drought stress tolerance in wheat

Fig. 5

Transient expression assays of TabHLH49. a Schematic representations of pCAMBIA1302 empty vector (control), pCAMBIA1302-TabHLH49:GFP (effector) and reporter constructs used in the transient expression system. The Nos box indicates the terminator. b Transactivation effect of TabHLH49 on the promoter of WZY2 (Pwzy2) shown by the dual-luciferase assay in tobacco leaves. The constructs described in (A) were transformed into Agrobacterium tumefaciens strain GV3101 and then infiltrated into tobacco leaves. The values of Rluc/Fluc are the mean ± SD from six samples, and significant differences are indicated as p < 0.05 (*) and p < 0.01 (**). (C-H) The phenotype (c), TabHLH49 and WZY2 gene expression levels (d-e), relative water content (f), chlorophyll content (g) and malondialdehyde content (h) of wheat leaves injected with Agrobacterium GV3101 containing pCAMBIA1302 empty vector (CK) and pCAMBIA1302:TabHLH49:GFP (OE) under normal growth conditions or drought stress conditions for 10 days. The values are the mean ± SD from three samples, and significant differences are indicated as p < 0.05 (*) and p < 0.01 (**)

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