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Fig. 1 | BMC Plant Biology

Fig. 1

From: CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

Fig. 1

Position of target sites of the sgRNAs in SlPMR4. a. Representation of genomic sequence of SlPMR4 showing the position of four single guide RNAs (sgRNAs) that were designed to produce knock-out mutants of SlPMR4. PCR primers Fw519 + Rv1925 and Fw2969 + Rv4230 were used to identify mutants. b. PROTTER [31] representation of the SlPMR4 protein. sgRNA6 (green) is located in the N-terminal FKS1dom1 domain of the protein; sgRNA8 (yellow), sgRNA1 (orange) and sgRNA7 (red) target sites in the intracellular part of the Glucan-synthase domain

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