CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

BMC Plant Biology

Table 2 Histological study of Oidium neolycopersici growth

Genotype	SlPMR4 mutation	Mutation event	Primary AP per IU (%)	Per AP		# Hyphae per IU (%)
Genotype	SlPMR4 mutation	Mutation event	Primary AP per IU (%)	Primary HS (%)	Primary HR (%)	1	2	3	4
MM	wild type	none	100	80	22	20	8	30	42
TV171009	bi-allelic	1 + 5	88	11.3^*	81.8	90	10	0	0
TV171010	homozygous	4	96	47.9	91.6^*	56	16	26	2
TV161212	bi-allelic	2 + 3	92	36.9	84.7^*	68	16	12	4

Development of Oidium neolycopersici on the susceptible genotype Moneymaker (MM) and three slpmr4 CRISPR mutant lines carrying different mutation events
Asterisks represent statistically significant differences between the mutant genotypes and cultivar MM as calculated by a t-test. *: p < 0.05
IU Infection unit, AP Appressorium, HS Haustorium, HR Hypersensitive response-like cell death

ISSN: 1471-2229