CRISPR/Cas9-mediated mutation of OsSWEET14 in rice cv. Zhonghua11 confers resistance to Xanthomonas oryzae pv. oryzae without yield penalty

Table 1 Disease resistance evaluation of Xoo strains from different regions. At least 10 leaves of three plants were inoculated for each Xoo strain

Strain^a	Strain origin	Lesion length and disease response^b
Strain^a	Strain origin	Zhonghua 11	CR S14–6^c
GD1358	China	8.11 ± 3.86 (MS)	0.26 ± 0.29 (R)
JS49–6	China	11.9 ± 2.50 (S)	0.87 ± 0.72 (R)
HB17	China	19.5 ± 8.23 (S)	1.74 ± 16.1 (R)
HB21	China	15.05 ± 5.85 (S)	0.14 ± 0.06 (R)
HLJ72	China	12.91 ± 4.87 (S)	0.65 ± 0.72 (R)
NX42	China	13.00 ± 7.24 (S)	0.63 ± 0.88 (R)
HN1–2	China	16 ± 5.62 (S)	0.7 ± 1.12 (R)
LC-4	China	17.05 ± 7.26 (S)	1.04 ± 1.25 (R)
IV-1	China	14.64 ± 5.36 (S)	0.43 ± 0.43 (R)
PXO79	Philippines	7.26 ± 2.86 (MS)	0.49 ± 0.42 (R)
PXO86	Philippines	8.17 ± 3.05 (MS)	0.25 ± 0.31 (R)
PXO71	Philippines	8.59 ± 2.94 (MS)	10.27 ± 2.44 (S)
Aust2031	Australia	8.78 ± 5.53 (MS)	0.24 ± 0.25 (R)
T7174	Japan	8.27 ± 8.44 (MS)	0.35 ± 0.28 (R)
A3857	India	10.57 ± 3.21 (S)	10.19 ± 3.61 (S)
A3842	India	10.11 ± 2.47 (S)	11.66 ± 2.48 (S)

^aXoo strains were cultivated two days on PSA medium and inoculated on six-week-old rice plants
^b Lesions length was scored 14 days after inoculation. R, resistant, lesion length < 3.0 cm; MS, moderately susceptible, 6.0 cm < lesion lenght≤9.0 cm; S, susceptible, lesion length > 9.0 cm
^cCR S14–6, one of the homozygous OsSWEET14 knockout mutant in Zhonghua 11 background

ISSN: 1471-2229