Fig. 3
H2O2 accumulation and cell death in different chickpea organs of control transgenic and CaWRKY70 overexpressing plants upon Foc1 infection. a, b DAB and trypan blue staining of chickpea roots under H2O2 treatment and Foc1 infection. c DAB and trypan blue colouration of leaves and stem portions from control transgenic and CaWRKY70 overexpressing chickpea plants. In a, b and c, chickpea plants were infected with Foc1 for 7 days. 10 mM H2O2 treatment was carried out for 30 min using chickpea roots. d Quantification of relative DAB staining activities in control transgenic and CaWRKY70 overexpressing chickpea roots (i), leaves (ii) and stem (iii) under control treatment Foc1 infection. (e) Quantitation of relative trypan blue colour intensities in control transgenic and CaWRKY70 overexpressing chickpea roots (i), leaves (ii) and stem (iii) under control treatment (0 dpi) and Foc1 stress. In d and e, error bars represent ±SD (n = 3). Asterisks (*) indicate values which are significantly different between control transgenic and CaWRKY70 overexpressing chickpea plants as determined by Student’s t test (*P ≤ 0.05 and **P ≤ 0.01)