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Fig. 7 | BMC Plant Biology

Fig. 7

From: Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition

Fig. 7

CaWRKY40 upregulates the activity of CaMPK9 promoter in planta. a In vivo binding of CaWRKY40 to CaMPK9 promoter at W-boxes. Schematic represents CaMPK9 promoter and the W-boxes relative to transcription start site (+ 1). Arrows indicate the sites for primer binding. In planta immunoprecipitation of WRKY40-chromatin complex from susceptible and resistant chickpea shoots with anti-CaWRKY40 antibodies. Bound chromatins were eluted and used for PCR reactions. Sheared pre-cleared chromatin served as input control. Rabbit IgG was used as negative control for ChIP PCR. b, c In vitro binding of recombinant WRKY40 to W-boxes at CaMPK9 promoter. Approximately, 200 ng of His-WRKY40 protein was added to W-box containing labeled CaMPK9 promoter fragment in an independent EMSA reaction. BP and FP indicate bound and free probes, respectively. Plus (+) and minus (−) signs denote presence or absence of individual elements. Asterisks highlight the W-boxes. Experiments were repeated twice with similar results. d Diagrams show 3’deletion fragments of CaMPK9 promoter and presence of the cis regulatory elements. e Genomic amplification of CaMPK9 promoter derivatives in transgenic tobacco by PCR. f Transactivation of pCaMPK9:GUS in transgenic N. tabacum seedlings upon transient expression of p35S:CaWRKY40

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