Skip to main content
Fig. 8 | BMC Plant Biology

Fig. 8

From: Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition

Fig. 8

CaWRKY70 attenuates R-protein signaling in chickpea. a BiFC assay for physical interaction between CC-NLR-nYFP and CaWRKY70-cYFP. (nYFP + CaWRKY70-cYFP) and (CC-NLR-nYFP + CaWRKY64-cYFP) interactions were used as negative and positive controls, respectively. N denotes nucleus. b H2O2 accumulation and cell death in chickpea leaves. Myc-CC-NLR, HA-WRKY64 and CaWRKY70 were transiently co-expressed in chickpea leaves by Agrobacterium. Infiltrated chickpea leaves were subjected to DAB and trypan blue staining. c Co-immunoprecipitation of c-myc-NB-ARC and CaWRKY70 after Agrobacterium mediated transient expression in N. benthamiana leaves. Arrow indicates the immunoprecipitated protein band of CaWRKY70. Asterisks show non-specific bindings. d His.-WRKY70 binding at W-box of chickpea EDS1 promoter by EMSA. Dotted lines represent W-box. BP and FP indicate bound and free probes, respectively. In b, c and d, plus (+) and minus (−) signs indicate presence or absence of specific components

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us