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Table 1 Summary of Illumina sequencing and assemblies for two J. vulgaris and two J. aquatica sets

From: Diversity and evolution of cytochrome P450s of Jacobaea vulgaris and Jacobaea aquatica

Sets a

Total paired-end clean reads

Total assembled trinity transcripts

Transcript length range

(nt b)

GC content (%)

Contig N50 c

(nt)

Average contig length (nt)

Reads mapped d (%)

Jv1*

19,725,242

152,286

301–13,238

39.37

1253

936

84.69

Jv2

36,359,675

142,213

301–13,269

39.31

1530

1132

83.25

Ja1*

20,306,518

118,936

301–15,708

39.27

1461

1082

91.57

Ja2

27,505,944

130,365

301–13,309

41.23

1441

1062

87.41

  1. a Jv1 consisted of the pooled shoots and roots of 59 individuals of J. vulgaris. Jv2 was composed of shoots from five tissue culture derived plants of J. vulgaris treated with MeJA and five mock treated individuals. Ja1 and Ja2 were derived from the same seven individuals of J. aquatica, of which roots were included in Ja1 but not in Ja2
  2. b nt: nucleotide
  3. c Contig N50: length such that sequence contigs of this length or longer include half the bases of the Trinity assembly
  4. d Reads mapped: the percentage of properly paired reads mapped back to the Trinity transcriptome assembly by Bowtie2
  5. * cDNA library was normalized before sequencing