Skip to main content
Fig. 1 | BMC Plant Biology

Fig. 1

From: TRANSTHYRETIN-LIKE and BYPASS1-LIKE co-regulate growth and cold tolerance in Arabidopsis

Fig. 1

B1L interacts with TTL. a Y2H analysis of the interaction between B1L and TTL. Each yeast clone containing TTL-pGADT7 (TTL-AD) or pGADT7 (AD) together with B1L-pGBKT7-B1L (B1L-BD) or pGBKT7 (BD) was grown on transformation selection (SD:/−W-L) or interaction selection (SD:/−W-L-H-A) plates. Dilution of the inoculation is shown at the top of the picture. Yeast growth on SD:/−L-T-H-A indicates a positive protein-protein interaction. b BiFC analysis in N. benthamiana showing the interaction between B1L and TTL. The N-terminal half of yellow fluorescent protein (YFPN) was fused to B1L (B1L-YFPN) and the C-terminal half of yellow fluorescent protein (YFPC) was fused to TTL (TTL-YFPC). The constructs were co-transformed into tobacco leaf cells, and fluorescence images were obtained by confocal microscopy. Panels from left to right show signals of yellow fluorescence, a bright-field image, and an overlay of yellow fluorescence and bright-field image images. Bar = 50 μm

Back to article page