Skip to main content

Table 1 List of oligonucleotides

From: Stimulation of homologous recombination in plants expressing heterologous recombinases

Oligonucleotidea

Restriction sitesb

Sequence (5′ - 3′)c

CaMVp-F

KpnI

GGGTACCCAAAGATTCAAATAGAGGACCT

rbcSL-R

HindIII; NdeI; NcoI

CCAAGCTTCCATATGAGCCATGGAAGCCATTTTTCTCAC

CaMVT-F

EcoRI

GGAATTCGTCCGCAAATCACCAGTCTCTC

CaMVT-R

SphI; SacI

CGCATGCGAGCTCGGTCACTGGATTTTGGTTTTAGG

NLS-F1

BamHI; SmaI

GATCCATGATGGGGACTCCTCCTAAGAAGAAGCGTAAGGTTCCC

NLS-R1

SmaI; BamHI

GGGAACCTTACGCTTCTTCTTAGGAGGAGTCCCCATCATG

NLS-F2

ApaI; SmaI

CATGATGGGGACTCCTCCTAAGAAGAAGCGTAAGGTTCCC

NLS-R2

ApaI; SmaI

GGGAACCTTACGCTTCTTCTTAGGAGGAGTCCCCATCATGGGCC

RecA-F

SmaI

TCCCCCGGGATGGCTATCGACGAAAACAAACAG

RecA-R

XbaI

GCTCTAGAAAAATCTTCGTTAGTTTCTGCTAC

RecG-F1

SmaI

TCCCCCGGGATGAAAGGTCGCCTGTTAGATGCTG

RecG-R1

XbaI

GCTCTAGACGCATTCGAGTAACGTTCCGTCTC

RecG-F2

HindIII

CCCAAGCTTGGGCCCATGATGGGGACTCC

RecG-R2

EcoRI

CGGAATTCTTACGCATTCGAGTAACGTTC

RuvC-F

SmaI

TCCCCCGGGATGGCTATTATTCTCGGCATTGATC

RuvC-R

PstI

GGCTGCAGTTAACGCAGTCGCCCTCTCGCCAGGTTCAG

Rad51-F

ApaI

GGGGCCCATGGCAATGCAGATGCAGCTTG

Rad51-R

PstI; SmaI

AACTGCAGCCCGGGTCAGTCTTTGGCATCTCCCAC

Rad52-F

SalI

GCGGTCGACATGTCTGGGACTGAGGAAGC

Rad52-R

SphI

GTAGCATGCGTAAGATGGATCATATTTCC

DMC1-F

ApaI

AAGGGCCCATGAAGGAGGATCAAGTTGTGGCG

DMC1-R

PstI; SphI

AACTGCAGGCATGCCTCCTTGGCATCCCCAATTCCTCC

  1. a F and R at the end of the oligonucleotide’s name indicate forward and reverse orientations, respectively. b The ends of annealed forward and reverse NLS primers are compatible for cloning into the indicated restriction sites. c The restriction sites are underlined in the sequence