Skip to main content
Fig. 7 | BMC Plant Biology

Fig. 7

From: Heat-response patterns of the heat shock transcription factor family in advanced development stages of wheat (Triticum aestivum L.) and thermotolerance-regulation by TaHsfA2–10

Fig. 7

The thermotolerance phenotypes, survival rate and the chlorophyll contents of atHsfA2–10 recovery Arabidopsis seedlings and WT under the normal conditions and HS. aTaHsfA2–10 relative expression in mutant (M), WT and three complementary lines of T3 generation by semi-RT PCR; b-c WT, athsfA2 mutant and its three TaHsfA2–10 complementary homozygous lines (16_30, 18_14 and 21_25) were used to assay the recovery thermotolerances. Five-day-old seedlings were treated with different HS regimes listed under each phenotype picture. After the seedlings were recovered at 22 °C for 8 days, the phenotypes were observed and photographed. After above, the survival rates (d) were counted and the rosettes leaves of each line were collected for measurement of chlorophyll contents (e). b-c seedlings under the normal conditions and HS; Total 50 individual plants of each line were divided into three parts and used for chlorophyll contents measurement; three plates were performed for each heat treatment. Each bar value represents mean ± SD of triplicate experiments; raw data refer to Additional file 2. Single and double asterisks indicate the significant differences between WT and overexpressing lines at P < 0.05 and P < 0.01 level (t-test), respectively

Back to article page