Fig. 7

Induction of anthocyanin accumulation in the leaves of GST1 genome-edited gentian lines. a Appearance of WT and GST1 genome-edited gentian plants on half-strength MS medium supplemented with 10% sucrose at 43 days after being transferred. b HPLC chromatograms at 365 nm of leaf extracts from plants grown on half-strength MS medium supplemented with 10% sucrose at 43 days after being transferred. The main peak is cyanidin 3-glucoside. c Anthocyanin contents in the leaves of the WT control and GST1 genome-edited lines. Quantities were determined based on the standard curve generated from the HPLC peak area of six serial dilutions of cyanidin 3-glucoside. Error bars represent the standard error of the means of five leaves from individual plants. Different letters indicate statistically significant differences according to Tukey’s HSD test (P < 0.01)