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Fig. 2 | BMC Plant Biology

Fig. 2

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Fig. 2

pigs-1 mutation disrupts pollen tube emergence and growth in vivo. a Gene Model of AtPIG-S (AT3G07180) with a T-DNA insertion (SAIL_162_D06) carrying the basta resistance and pLAT52:GUS genes. Black rectangles indicate exons and lines indicate introns and UTRs. Basta resistance gene is under the control of the 35S promoter and a post meiotic, pollen-specific promoter of LAT52 drives the beta-glucronidase reporter (GUS) gene. Both junctions of T-DNA insertion sites in the AtPIGS gene were sequenced to identify the insertion point in the second intron, which caused a 34 bp deletion. b-c Anther locules containing indehiscent pollen tetrads in pigs-1/+ (b) or wild-type backgrounds (c) were stained with X-Gluc for GUS activity. Scale bar 100 uM. d-e Released pollen tetrads from (d) pigs-1/+ or (e) wild-type anthers show indistinguishable morphology and GUS expression (n = 100, in each genotype). Scale bar 50 uM. f Stigma of wild-type pistil pollinated with pigs-1/+ stained for GUS activity 18 h after pollination (HAP). Black arrowhead, pigs-1 pollen showing pollen tube emergence defect. Scale bar 100 uM. g Rare instance of pigs-1 pollen tube approaching wild-type ovule within a wild-type pistil pollinated with pigs-1/+ and stained for GUS activity 18 HAP. pigs-1 pollen tube grew along the funiculus and failed to target the micropyle. Black arrowhead, pigs-1 pollen tube growing away from the micropyle. Scale bar 100 uM. h-j Light micrograph of wild-type pistil pollinated with either (h) pLAT52:GUS/+, (i) pigs-1/+, or (j) pigs-1/+ transformed with pPIGS:GFP-PIGS line #15–3 pollen and stained for GUS activity 18 HAP. Scale bar 300 uM

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