AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Table 1 Reduced transmission of the pigs-1 and gpi8–2 mutations through the male gametophyte

Female parent	Male parent	Observed No. of progeny		TE (R/S)	Χ²	P-value
Female parent	Male parent	Basta^Ra	Basta^Sa	TE (R/S)	Χ²	P-value
BastaR/+	Wild type	120	131	0.92	0.48	0.49
lre-7/+	Wild type	29	181	0.16	110	< 0.001
gpi8–2/+	Wild type	177	169	1.05	0.19	0.67
pigs-1/+	Wild type	255	268	0.95	0.32	0.57
Wild type	BastaR/+	189	193	0.98	0.04	0.84
Wild type	gpi8–2/+	0	607	0	607	< 0.001
Wild type	pigs-1/+	8	483	0.02	460	< 0.001
Wild type	pigs-1/+; GFP-PIGS	108	142	0.76	4.62	0.03

Wild type, Columbia-0 ecotype
TE, Transmission efficiency was calculated as the quotient of the number of basta resistant (R) divided by basta susceptible (S) progeny of the indicated cross
χ2 was calculated based on the expectation of a 1:1 segregation of basta resistance to susceptibility in the progeny. GFP-PIGS refers to the T2 line of pPIGS:GFP-PIGS #15–3
^aBasta resistant (Basta^R) and Basta susceptible (Basta^S) progeny. Basta resistance gene is linked with the T-DNA that is inserted into the AtGPI8 (gpi8–2) [25] and LRE (lre-7), AtPIGS (pigs-1), and in an unknown location in the genome of the BastaR/+ line [21]

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