Fig. 5
From: OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice
OsCAF2 interacts with OsCRS2 through the C-terminal region. a The Y2H interaction between OsCAF2 and OsCRS2. The full-length CDS of OsCAF2 and OsCRS2 were cloned into pGBKT7 (BD) and pGADT7 (AD) vectors to produce OsCAF2-BD, OsCAF2-AD, OsCRS2-BD, and OsCRS2-AD plasmids, respectively. The OsCAF2-BD and OsCRS2-AD, OsCAF2-AD and OsCRS2-BD were co-transformed into the AH109 yeast strain. The OsCAF2-BD and AD plasmid, and OsCRS2-AD and BD plasmid were co-transformed into the AH109 yeast strain as a control. b Schematic diagram of OsCAF2 protein structure. Different sections of OsCAF2 involving in OsCAF2-N (1–230 aa), OsCAF2-M (231–430 aa), and OsCAF2-C (431–608 aa). c Y2H interaction of OsCRS2 and different truncated sections of OsCAF2. The truncated sections of OsCAF2 were cloned into the BD vector to generate OsCAF2-N-BD, OsCAF2-M-BD, and OsCAF2-C-BD vectors. These three vectors were co-transferred into AH109 yeast cells with the OsCRS2-AD vector. The yeast cells were cultured on SD-T/L and SD−/T/L/H/A media with different dilution series (1, 10− 1, and 10− 2)