Table 1 Analysis of male development in di- and polyploid Ranunculus gametes during sporogenesis. Mean percentages of normal and abnormal sporogenesis were determined by orcein staining and bright field microscopy
Taxa | Ploidy | Plant ID | n (normal, abnormal) | normal sporogenesis (range) | abnormal sporogenesis (range) |
|---|---|---|---|---|---|
Parent species | |||||
 R. notabilis | 2x | 10137, 9609 | 923 (916, 7) | 0.99 (0.50–0.99) | 0.02 (0.01–0.50) |
 R. carpaticola | 2x | 8483, LH040 | 369 (365, 4) | 0.97 (0.94–0.99) | 0.03 (0.01–0.05) |
 R. cassubicifolius | 4x | LH008 | 324 (314, 10) | 0.97 (0.96–0.98) | 0.03 (0.02–0.04) |
Synthetic F1 Hybrids | |||||
 R. carpaticola * R. notabilis | 2x | J, F | 3154 (3123, 31) | 0.99 (0.99–1.00) | 0.01 (0.00–0.01) |
 R. cassubicifolius * R. notabilis | 3x | G | 645 (615, 30) | 0.89 (0.79–0.99) | 0.11 (0.11–0.21) |
Synthetic F2 Hybrids | |||||
 R. car. * R. not. * R. car. * R. not. | 2x | F x F, F x J, J x F, J x J | 3653 (3587, 66) | 0.98 (0.95–1.00) | 0.03 (0.00–0.17) |
 R. cas. * R. not. * R. cas. * R. not. | 3x, 4x | G x G | 211 (181, 30) | 0.86 (0.76–0.95) | 0.14 (0.05–0.24) |
Natural Hybrids | |||||
 R. notabilis * R. variabilis (?) | 4x | 10136 | 1001 (914, 87) | 0.82 (0.50–0.99) | 0.18 (0.01–0.50) |
 Diploid Samples |  |  | 8099 (7991, 108) | 0.98 (0.83–1.00) | 0.02 (0.00–0.17) |
 Polyploid Samples |  |  | 2181 (2024, 157) | 0.87 (0.50–0.99) | 0.13 (0.01–0.50) |
 Total |  |  | 10,280 (10,015, 265) | 94.58% | 5.42% |